StressMarq明星产品DNA损伤试剂盒新年发表文献

         8-OH-dG (8-hydroxy-2-deoxy Guanosine) 是DNA受活性氧及活性氮等作用造成氧化损伤的产物，是氧化应激的标记物。鸟苷的羟基化可发生于正常的新陈代谢进程或者受外界环境因素影响时。8-OH-dG的增多通常还与一些疾病有关，例如癌症，糖尿病，高血压等。
         在一些复杂的样品中，例如血浆，细胞裂解液，以及组织中，8-OH-dG既可以以游离的形式存在也可以以整合于DNA中的形式存在。一旦血液进入肾脏当中，游离的8-OH-dG就会渗出到尿液中，而大分子的DNA碎片则留在血流里。由于血浆样品的复杂性，尿液是更好的用来检测游离8-OH-dG的样品。尿液样品中的8-OH-dG水平大概是2.7~13 ng/mg creatine，而血浆中的游离8-OH-dG水平是4~21 pg/ml。

  
       
       StressMarq的DNA损伤ELISA试剂盒是采用的竞争法来定量检测尿液、细胞培养基、血浆和其他样品中的8-OHdG。竞争法ELISA更适用于检测小分子物质，检测结果比夹心法要准确， 数据再现性很高，这使该试剂盒有更高的灵敏度和更广的检测范围。


         我们近两年改进了我们的试剂盒，升级版DNA损伤试剂盒SKT-120-96s/SKT-120-480s与旧款试剂盒核心抗体一致，加强了试剂的稳定性，调整了实验方案，达到旧款到新款的无缝转换，而价格依旧实惠。
产品特点:
1. 检测时间更短 – 新试剂盒的检测时间由原来的18小时缩短到仅仅1个小时。
2. 稳定性更强 – 旧款试剂盒的乙酰胆碱酯酶和 Ellmans’ 试剂盒被替换成 HRP 以及 TMB，增强了稳定性，无需储存在 -20°C。
3. 简化的实验步骤 – 直接标记的一抗以及其他组件的改进减少了所需试剂，从而简化了实验步骤。
4. 更广的检测范围 – 新试剂盒检测范围增加到 0.94 - 60 ng/ml ，这可以减少稀释步骤并且该范围可能更适宜生物样品的读数。

新旧试剂盒数据比较:                              

	旧款 SKT-120-96	新款 SKT-120-96S
孵育时间	18 小时	1 小时
灵敏度	100 pg/ml	0.59 ng/ml
检测范围	40 – 1.2 x 103 pg/ml	0.94 – 60 ng/ml
样品量/试剂盒:	40 in duplicate 24 in triplicate	39 in duplicate 23 in triplicate

 
 
 
试剂盒最新发表文献：
The biological age linked to oxidative stress modifies breast cancer aggressiveness.
Saez Freire, M.D.M. et al. (2018) Free Radic Biol Med. [Epub ahead of print].
PubMed ID: 29550329         适用物种: Mouse              适用样本: Liver
 
SOCS1-targeted therapy ameliorates renal and vascular oxidative stress in diabetes via STAT1 and PI3K inhibition.
Lopez-Sanz, L. et al. (2018) Lab Invest. [Epub ahead of print].
PubMed ID: 29540859        适用物种: Mouse              适用样本: Blood and urine
 
Effects of ozone repeated short exposures on the airway/lung inflammation, airway hyperresponsiveness and mucus production in a mouse model of ovalbumin-induced asthma.
Bao, W. et al. (2018) Biomed Pharmacother. [Epub ahead of print].
PubMed ID: 29499403         适用物种: Mouse            适用样本: Lung
 
Ginsenoside Rb1 prevents high glucose-induced Schwann cell injury through the mitochondrial apoptosis pathway.
J Tradit Chin Med. 37(6): 746-755. (2018). Li, X. et al.
PubMed ID: N/A                   适用物种: Human          适用样本: Schwann Cells
 
Urinary 8-hydroxydeoxyguanosine is a better biomarker of aging in non-smokers.
Tradit. Med. Mod. Med. (2018). Zhao, Z.X. et al.
PubMed ID: N/A                   适用物种: Human         适用样本: Urine
 

IL-6 receptor blockade ameliorates diabetic nephropathy via inhibiting inflammasome in mice. Metabolism. [Epub ahead of print]. (2018). Wu, R. et al. PubMed ID: 29336982.      适用物种: Mouse.        适用样本: Urine

Analysis of Oxidative DNA Damage in HIV-Positive Pregnant Women. IJIRMS. 02(10):2455-8737. (2017). Rajekar, R.B. PubMed ID: N/A.                 适用物种: Human.       适用样本: Plasma.

Gene expression, oxidative stress and senescence of primary coronary endothelial cells exposed to postprandial serum of healthy adult and elderly volunteers after oven cooked meat meals. Mediators Inflamm.  [Epub ahead of print]. 2017. Laura, C. et al. PubMed ID: N/A.                 适用物种: Human.       适用样本: HCAECs.

A multi-biomarker analysis of the antioxidant efficacy of Parkinson's disease therapy. Toxicol In Vitro. [Epub ahead of print]. 2017. Colamartino, M. et al. PubMed ID: 29080800.     适用物种: Human.        适用样本: Blood buffy coat.

Association of Metabolic Syndrome and Oxidative DNA Damage in HIV/AIDS Patients. Ind J Clin Biochem. [Epub ahead of print]. 2017. Kolgiri, V., Nagar, V. and Patil, V. PubMed ID: 28535982.     适用物种: Human.        适用样本: Plasma.

Antiretroviral Therapy‑induced Insulin Resistance and Oxidative Deoxy Nucleic Acid Damage in Human Immunodeficiency Virus‑1 Patients. Indian J Endocr Metab. 21(2):316-321. 2017. VK Honnapurmath, V.W.P. PubMed ID: 28459032.     适用物种: Human.        适用样本: Blood.

 
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